LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2258..2354) /label=attL2 /note="attL2 (c)" misc_signal complement(2369..2388) /note="T7 Promoter/priming site (c)" primer_bind 2396..2412 /note="M13 Reverse priming site" gene 2525..3334 /note="Kanamycin resistance gene" rep_origin 3455..4128 /note="pUC origin" PCR_frame 669..2258 /note="Reading Frame" /set="Set 1" PCR_primer 652..668 /primer="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original=ACAAGTTTGTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC PCR_primer complement(669..2278) /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original=ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG PCR_product complement(652..2275) /pair="Pair 1" /set="Set 1" /current=1 /score=1.000 vector join(2266..4131,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 2236..2256 /note="TEV site" source 673..2235 /organism="synthetic construct" /mol_type="other DNA" /db_xref="taxon:32630" /clone="MGC:195833 IMAGE:100068216" /clone_lib="NIH_MGC_435" /lab_host="DH10B" /focus /note="Vector: pENTR223.1 with stop codon" source 673..2235 /organism="Homo sapiens" /mol_type="other DNA" /db_xref="taxon:9606" gene 673..2235 /gene="PDIA2" /gene_synonym=PDA2 /gene_synonym=PDI /gene_synonym=PDIR /db_xref="GeneID:64714" /db_xref="HGNC:14180" /db_xref="MIM:608012" CDS 673..2235 /dnas_title="protein disulfide isomerase family A, member 2" /gene="PDIA2" /gene_synonym=PDA2 /gene_synonym=PDI /gene_synonym=PDIR /codon_start=1 /product="protein disulfide isomerase family A, member 2" /protein_id="AAI67826.1" /db_xref="GI:189442867" /db_xref="GeneID:64714" /db_xref="HGNC:14180" /db_xref="MIM:608012" /translation="MSRQLLPVLLLLLLRASCPWGQEQGARSPSEEPPEEEIPKEDGI LVLSRHTLGLALREHPALLVEFYAPWCGHCQALAPEYSKAAAVLAAESMVVTLAKVDG PAQRELAEEFGVTEYPTLKFFRNGNRTHPEEYTGPRDAEGIAEWLRRRVGPSAMRLED EAAAQALIGGRDLVVIGFFQDLQDEDVATFLALAQDALDMTFGLTDRPRLFQQFGLTK DTVVLFKKFDEGRADFPVDEELGLDLGDLSRFLVTHSMRLVTEFNSQTSAKIFAARIL NHLLLFVNQTLAAHRELLAGFGEAAPRFRGQVLFVVVDVAADNEHVLQYFGLKAEAAP TLRLVNLETTKKYAPVDGGPVTAASITAFCHAVLNGQVKPYLLSQEIPPDWDQRPVKT LVGKNFEQVAFDETKNVFVKFYAPWCTHCKEMAPAWEALAEKYQDHEDIIIAELDATA NELDAFAVHGFPTLKYFPAGPGRKVIEYKSTRDLETFSKFLDNGGVLPTEEPPEEPAA PFPEPPANSTMGSKEEL" misc_feature 673..2235 /note="PDIA2 coding region" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTCG CCatgagccg ccagcttctg cctgtactgc tgctgctgct gctcagggct 721 tcgtgcccat ggggtcagga acagggagcg aggagcccct cggaggagcc tccagaggag 781 gaaatcccca aggaggatgg gatcttggtg ctgagccgcc acaccctggg cctggccctg 841 cgggagcacc ctgccctgct ggtggaattc tatgccccgt ggtgtgggca ctgccaggcc 901 ctggcccccg agtacagcaa ggcagctgcc gtgctcgcgg ccgagtcaat ggtggtcacg 961 ctggccaagg tggatgggcc cgcgcagcgc gagctggctg aggagtttgg tgtgacggag 1021 taccctacgc tcaagttctt ccgcaatggg aaccgcacgc acccggagga gtacacagga 1081 ccacgggacg ctgagggcat tgccgagtgg ctgcgacggc gggtggggcc cagtgccatg 1141 cggctggagg acgaggcggc cgcccaggcg ctgatcggtg gccgggacct agtggtcatt 1201 ggcttcttcc aggacctgca ggacgaggac gtggccacct tcttggcctt ggcccaggac 1261 gccctggaca tgacctttgg cctcacagac cggccgcggc tctttcagca gtttggcctc 1321 accaaggaca ctgtggttct cttcaagaag tttgatgagg ggcgggcaga cttccccgtg 1381 gacgaggagc ttggcctgga cctgggggat ctgtcgcgct tcctggtcac acacagcatg 1441 cgcctggtca cggagttcaa cagccagacg tctgccaaga tcttcgcggc caggatcctc 1501 aaccacctgc tgctgtttgt caaccagacg ctggctgcgc accgggagct cctagcgggc 1561 tttggggagg cagctccccg cttccggggg caggtgctgt tcgtggtggt ggacgtggcg 1621 gccgacaatg agcacgtgct gcagtacttt ggactcaagg ctgaggcagc ccccactctg 1681 cgcttggtca accttgaaac cactaagaag tatgcgcctg tggatggggg ccctgtcacc 1741 gcagcgtcca tcactgcttt ctgccatgca gtcctcaacg gccaagtcaa gccctatctc 1801 ctgagccagg agataccccc tgattgggat cagcggccag ttaagaccct cgtgggcaag 1861 aattttgagc aggtggcttt tgacgaaacc aagaatgtgt ttgtcaagtt ctatgccccg 1921 tggtgcaccc actgcaagga gatggcccct gcctgggagg cattggctga gaagtaccaa 1981 gaccacgagg acatcatcat tgctgagctg gatgccacgg ccaacgagct ggatgccttc 2041 gctgtgcacg gcttccctac tctcaagtac ttcccagcag ggccaggtcg gaaggtgatt 2101 gaatacaaaa gcaccaggga cctggagact ttctccaagt tcctggacaa cgggggcgtg 2161 ctgcccacgg aggagccccc ggaggagcca gcagccccgt tcccggagcc accggccaac 2221 tccactatgg ggtccgaaaa cttgtacttt caaggctCCA GCTTTCTTGT ACAAAGTTGG 2281 CATTATAAGA AAGCATTGCT TATCAATTTG TTGCAACGAA CAGGTCACTA TCAGTCAAAA 2341 TAAAATCATT ATTTGCCATC CAGCTGATAT CCCCTATAGT GAGTCGTATT ACATGGTCAT 2401 AGCTGTTTCC TGGCAGCTCT GGCCCGTGTC TCAAAATCTC TGATGTTACA TTGCACAAGA 2461 TAAAATAATA TCATCATGAA CAATAAAACT GTCTGCTTAC ATAAACAGTA ATACAAGGGG 2521 TGTTATGAGC CATATTCAAC GGGAAACGTC GAGGCCGCGA TTAAATTCCA ACATGGATGC 2581 TGATTTATAT GGGTATAAAT GGGCTCGCGA TAATGTCGGG CAATCAGGTG CGACAATCTA 2641 TCGCTTGTAT GGGAAGCCCG ATGCGCCAGA GTTGTTTCTG AAACATGGCA AAGGTAGCGT 2701 TGCCAATGAT GTTACAGATG AGATGGTCAG ACTAAACTGG CTGACGGAAT TTATGCCTCT 2761 TCCGACCATC AAGCATTTTA TCCGTACTCC TGATGATGCA TGGTTACTCA CCACTGCGAT 2821 CCCCGGAAAA ACAGCATTCC AGGTATTAGA AGAATATCCT GATTCAGGTG AAAATATTGT 2881 TGATGCGCTG GCAGTGTTCC TGCGCCGGTT GCATTCGATT CCTGTTTGTA ATTGTCCTTT 2941 TAACAGCGAT CGCGTATTTC GTCTCGCTCA GGCGCAATCA CGAATGAATA ACGGTTTGGT 3001 TGATGCGAGT GATTTTGATG ACGAGCGTAA TGGCTGGCCT GTTGAACAAG TCTGGAAAGA 3061 AATGCATAAA CTTTTGCCAT TCTCACCGGA TTCAGTCGTC ACTCATGGTG ATTTCTCACT 3121 TGATAACCTT ATTTTTGACG AGGGGAAATT AATAGGTTGT ATTGATGTTG GACGAGTCGG 3181 AATCGCAGAC CGATACCAGG ATCTTGCCAT CCTATGGAAC TGCCTCGGTG AGTTTTCTCC 3241 TTCATTACAG AAACGGCTTT TTCAAAAATA TGGTATTGAT AATCCTGATA TGAATAAATT 3301 GCAGTTTCAT TTGATGCTCG ATGAGTTTTT CTAATCAGAA TTGGTTAATT GGTTGTAACA 3361 CTGGCAGAGC ATTACGCTGA CTTGACGGGA CGGCGCAAGC TCATGACCAA AATCCCTTAA 3421 CGTGAGTTAC GCGTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC 3481 TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC 3541 AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT 3601 CAGCAGAGCG CAGATACCAA ATACTGTTCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT 3661 CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC 3721 TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA 3781 GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC 3841 CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG 3901 GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA 3961 GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT 4021 TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA 4081 CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT T //